Original article
English
Mohammed A. A. Khadre
Department of Microbiology & Parasitology, Faculty of Medicine, University of Al Fateh, Tripoli, The Socialist People’s Libyan Arab Jamahiriya.
Al Fateh Med. J Vol .2, No. 1. July 1982:17-20.
Abstract
A new method for detection of Mycobacterium tuberculosis in sputum specimens is presented. In a group of 176 sputum specimens obtained from patients suspected of being sick with tuberculosis, 152 (86.4°c) were found to be positive for acid fast bacilli (AFB) using our new method. On the other hand, the direct staining Ziehl-Neelsen method and the cultivation on Lowenstein Jensen medium detected acid fast bacilli only in 58 and 69.9°/a respectively. Furthermore, the new method still detected AFB in some sputum specimens diluted up to 1/2048 while the Ziehl-Neelsen staining method failed to do so after 1/128 and the culture method was negative after 1/256 dilutions. The significance of these results is discussed.
Keywords: A New Method for Detection of Tubercie Bacilli in Sputum Specimens
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