Elyousfi Masaoud A., Ramadan Abdoalbast, Tbeny Nabil, Alaiwany Narges, Almagerhy Reem.
Department of laboratories, Faculty of Medical Technology Al Fatah University-Tripoli, Libya
Garyounis Medical Journal Vol. 21, No.1. 2004:88-92
Background: A major problem in antibiotic therapy is the emergence of drug resistant bacteria; the frequency of resistance depends on the bacteria, and the antibiotic concerned. Delivery devices such as forceps, and vacuum extractors are well known devices that carry health risks during delivery. The total deliveries within the Tripoli Medical Center MC), between January 1998 and December 2001, were 39827 births (alive and dead; stillbirths are not included). Objective: 1- to isolate and identify bacteria from different samples within the delivery moms and delivery department, in order to evaluate the extent of contamination within such a department 2- to determine the susceptibility of bacterial isolates to several antibiotics using the disk-diffusion method . This bacteriological study was conducted between October 1999 and June 2001 during 5 interval visits to the delivery moms and delivery department at (TMC). Materials and Methods: Several duplicate samples were taken from the delivery department and examined using standard bacteriological procedures. Results: 17 bacterial isolates, mostly gram negative aerobic, and facultative anaerobic bacilli, were isolated from a total of 90 samples within the department and delivery rooms. Several bacterial isolates, were found to be resistant to more than one of the antibiotics, that are used commonly for treating bacterial diseases. The most frequent bacterial isolates, which are considered to be important bacteria in hospital acquired infections, were Staphylococcus aureus (44.4%), Staphylococcus epidermidis (24.4%), and Pseudomonas aeruginosa (12.2%). The most frequent contaminated samp1es, were those taken from the delivery beds. Conclusion: This study demonstrated that the number of bacterial isolates from the delivery rooms, and the delivery department was high. Furthermore, the delivery moms, and the delivery department should be disinfected periodically. Since most bacterial isolates, were highly resistant to several antibiotics tested, therefore, alternative drags must be used and a restrictive policy on their proper use is important to prevent the spread of antibiotic resistance within (TMC).
Keywords: Nosocomial infection , antibiotic resistance, gram negative bacteria, S. arneus, S. epidermidis.