Original article
English
Abdul H . Khan 1, Mohammed A . Al-Fellani 1, Muftah Y. Ali 2; Yosef Kabti 2, Abdunnaser A . El-Buni 3
1-Department of Parasitology , Faculty of Medicine, Sebha University, Sebha, Libya. 2-Department of Microbiology and Parasitology, Faculty of Medicine, Garyounis University, Bengahzi, Libya. 3-3-Department of Parasitology , Faculty of Medicine, Al-Fateh University, Tripoli, Libya.
Garyounis Medical Journal Vol. 22, No.1. 2005:22-26
Abstract
Background: Hydatid disease caused by larva of Echinococcus granulosus is endemic and poses a significant economic and public health problem in Libya.
Objectives: To improve the sensitivity and reproducibility of serological tests for the diagnosis of human cystic echinococcosis (EC) by using locally available hydatid antigen.
Materials and Methods: Enzyme linked immunosorbent (ELISA) and indirect haemagglutination (IHA) tests were employed to detect anti-hydatid antibodies in the serum of 30 surgically confirmed hydatid patients, 50 clinically suspected hydatidiosis patients and 20 normal healthy subjects.
Results: Somatic hydatid antigens produce significantly greater OD values (P<0.05) compared with different cyst-fluid antigens and showed higher reproducibility for the diagnosis of human CE in ELISA than
IHA.
Conclusion: To avoid false seronegative results in surigically confirmed and clinically suspected hydatid patients, use of E.granulosus antigens from local source in serological techniques is recommended, which would give better sensitivity.
Keywords: ELISA, cystic echinococcosis. somatic-antigen
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