Characterisation of Echinococcus Granulosus Hydatid Antigens by Human IgG-Subclasses and IgE

Original article


Ali O Daeki¹, Philip S Craig², Salaheddin M Gashout³

1-Department of Microbiology and Immunology, Faculty of Medicine, University of Al-Fateh, Tripoli 2-Department of Biological Sciences, University of Salford, Salford M54 WT, UK 3-Burns and plastic surgery Centre, Tripoli, Libya

JMJ Vol. 7, No. 2 (Summer 2007): 87-96


Analysis of E. granulosus protoscolex extract and cyst fluid by SDS-PAGE indicated some different antigenic components between these two sources of antigens. These were at considerably greater concentration in the protoscolex extract, and may indicate, a better source of antigens in comparison with hydatid cyst fluid. Relatively the same E. granulosus protoscolex antigens molecular weights were, with relative different molecular weights of approximately 200, 180, 93, 76, 67, 45, 40, 30, 20, 15 and 10 kDa, detected by immunoblotting using Cystic Hydatidosis patients sera. In accordance with previous results using hydatid cyst fluid antigens, these immunoblotting results indicate that IgG1, IgG3, IgG4 and IgE antibodies in Cystic Hydatidosis patient recognising different antigens of E. granulosus. Antibodies in the subclass IgG1 or IgG3 and IgE antibodies strongly recognized the 24, 38-40, 48 and 59kDa AgB and Ag5 subunits antigens, where IgG4 inaddition recognizes the smallest AgB subunit (8kDa). This study also indicates that the 24 kDa is an AgB subunit but not Ag5 and the 38kDa is the small Ag5 subunit. It also revealed remarkably different binding profiles between IgG1 and IgG4 antibodies to hydatid cyst fluid antigens. Imaging methods do not always indicate clear changes in cyst viability and combining the ELISA and immunoblotting techniques used in this study therefore provide additional or supporting evidence for good clinical diagnosis for Cystic Hydatidosis patients.

Keywords: Libya, IgG subclasses, IgE, Cystic Hydatidosis, E. Granulosus.