Chromosomal localization of Human Microsomal Epoxide Hydrolase gene

Original article


I.M. Ateitalla 1, S. Brown 2

1- Biochemistry Department, Faculty of Medicine, Garyounis University, Benghazi, Libya 2-Department of Biochemistry, Medical School, Nottingham University, Nottingham, UK.

Garyounis Medical Journal Vol.20,No.1.2003:37-44


Objectives :Epoxide hydrolase is an enzyme involved in the metabolism of the carcinogen benzo[a] pyrene. It converts reactive arene oxides into dihydrodiols. This can be considered either as a part of an activation process or as a part of a detoxification process with respect to benzo[a]pyrene metabolism. Initially the human microsomal epoxide hydrolase gene was mapped to chromosome (6) and then a contradicting report mapped it to chromosome (1). Thus it was deemed necessary to confirm its chromosome location. Materials and methods: Primers were produced which could amplify a region of the human gene and were then used to test a series of hybrid clones for the presence or absence of the gene. The data obtained was correlated with the presence or absence of each human chromosome Results and conclusion: it was found that the human microsomal epoxide hydrolase gene segregated with chromosome (1) markers confirming one of the previous assignments.

Keywords: Epoxide Hydrolase; Hybrid cells; PCR.