Collagen synthesis by human bone marrow stromal cells derived from acute myeloid leukaemia patients and from normal donors

Original article


R Nafu, M Nemeth- cs oka – Lelkes Gabor

National Institute of Haematology and Blood Transfusion Budapest Hungary

JMJ Vol. 2, No. 2 (September 2002): 61-64


The bone marrow stroma, represented in human bone marrow cultures by cells of the adherent layers, is composed of fibroblasts, macrophages, fat cells, endothelial like cells and it is associated with extracellular matrix (ECM). This ECM is capable of binding haemopoietic growth factors and plays a major role in regulation of haematopoiesis. A series of marrow stroma derived from patients with acute myeloid leukaemia (AML) and from normal donors were investigated for their properties of collagen synthesis. The stromal cells were separated and the calls were disrupted using a sonicatior, then cellular proteins were precipitated by ammonium sulphate and digested with pepsin and collagens were separated by SDS-8% polyacrylamide gel electrophoresis. Both collagen types I and III were present in normal stroma and stroma derived from AML patients as well. On the other hand the band, which migrated on polyacrylamid gel electrophoresis and was seen above 2a chain of collagen type I was identified only in the stroma derived from patients with AML. The reason for this different finding might reflect the abnormalities of haemopoietic microenvironment of acute myeloid leukaemia in vivo.

Keywords: extracellular matrix (ECM), Haemopoietic growth factors, Haematopoiesis