Original article
English
Rabia Nafu, Gabor Lelkes, Julia Gidali, Gyôzô Petrلnyi
National Institute of Haematology and Blood Transfusion ,Budapest, Hungary.
Garyounis Medical Journal Vol. 18. 1995-1997: 90-105
Abstract
Cultured bone marrow stroma derived from normal donors and from patients with various haematological disease such as Acute Myeloid Leukemia (AML), Acute Lymphoblastic Leukemia (ALL), Chronic Myeloid Leukemia (CML), Myelodysplastic Syndrome (MDS) and p1astic Anemia (AA), was studied ultrastructurally for its cellular composition. The stroma was composed of myofibroblasts cells, macrophage like cells, fat cells, endothelial-like cells and blanket cells. Gap junctions were occasionally bound between adherent stromal cells.
In the course of studying some properties of the normal and abnormal adherent human bone marrow stromal cells, it was observed that a small number of adherent cells phagocytosed atex particles. FC receptors were seen on the surface of a small number of adherent cells. The idhesive glycoproteins, laminin and fibronectin were detected ultrastucturally by using the immunoperoxidase staining technique with specific anti-sera against these matrix components. rhe former was also examined by immunoelectrophoresis. It was concluded from that the stroma derived from patients with various haematological diseases does not show significant ifference in cell composition and the laminin in the extracellular matrix, indicated indirectly the presence of Endothelial cells in the samples.
Keywords: Comparative Morphological Study on the Ultrastucture of Cultured Adherent Cells from Normal and Abnormal Human Bone Marrow Samples.
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