Cysteine-dependent uropathogens: Isolation, identification and susceptibility to antimicrobial agents

Original article


Tarek M. Gibreel1, Khalifa Sifaw Ghenghesh 2

1) Central Hospital Laboratories, 2) Department of Medical Microbiology, Faculty of Medicine, Elfateh University, Tripoli, Libya

JMJ Vol. 2, No. 2 (September 2002): 52-54


Members of the family Enterobacteriacea that are auxotrophic for cysteine because of defects in the sulfur assimilatory pathway account for up to 2 % of significant isolates from urinary tract infections. In the present work bacterial isolates from Libyan patients with urinary tract infections were examined for cysteine – dependence and susceptibility to antibiotics. Methods of isolation, identification and antimicrobial susceptibility testing were used in presence and absence of cysteine supplementation Of 675 enterobacterial isolates examined, nearly 1.5% were cysteine – dependent uropathogens (CDUP). A significant association of CDUP with abnormal urological conditions was observed. The means by which laboratory procedures can be adapted to fully identify such organisms are simple and should by used whenever auxortrophy is expected.

Keywords: Enterobacteriacea, Cystein, Cysteine-dependent uropathogens (CDUP).