Original article
English
Muna Mohamed Buzayan 1, Rajab Salem Tobgi 2, Ibrahim Taher 2
1-Libyan National Centre of Infectious Disease Prevention and Control Branch of Benghazi, Bacteriology Laboratory Unit. 2- Department of Microbiology, Faculty of Medicine, Garyounis University Benghazi, Libya
JMJ 2010,Vol.10, No.1 , 10-16
Abstract
Objective: This study was conducted to determine the prevalence of extended spectrum ك lactamases (ESBL) production among urinary isolates of E. coli and Klebsiella pneumoniae at two centres in Benghazi and to assess the effect of 22 antibiotics on these bacterial isolates. Methods: A total of 169 clinical isolates representing 105 E. coli and 42 K. pneumoniae isolated from HIV-negative outpatients, fourteen E. coli and eight K. pneumoniae obtained from HIV-positive patients were included in this study. All isolates were collected from patients diagnosed with urinary tract infection. The Kirby Bauer disk diffusion method, double disk synergy test and disk replacement methods were used. Results: All 169 strains of E. coli and K. pneumoniae were sensitive to imipenem and amikacin (100%). The rates of resistance of E. coli to nalidixic acid and ciprofloxacin (22.9%, 17.1%) among HIV negative outpatients were higher than HIV positive patients (7.1%, 7.1%) respectively. Chloramphenicol showed the same resistant pattern in both HIV negative and positive patients (14.3%) whereas all K. pneumoniae isolates were more resistance to ampicillin and ticarcillin (100%, for both) among HIV positive compared to HIV negative patients (100%,85.7%). Of 119 strains of E. coli and 50 strains of K. pneumoniae tested for ESBLs production, only five strains were ESBL’s positive; one K. pneumoniae from HIV negative patient (2.4%) and four E. coli strains; two were isolated from HIV negative (1.9%) and two from HIV positive patients (14.3%). Conclusion: This study revealed that ESBLs producing isolates seems to be more widespread in HIV positive patients (E.coli 14.3%) than among HIV negative outpatients (E. coli 1.9% and K. pneumoniae 2.4%).
Keywords: Antibiotic resistance, ESBL-producing, E. coli, K. pneumoniae, DDST, HIV negative outpatients, HIV positive patients.
Link/DOI: http://www.jmj.org.ly/images/stories/spring2010/10.pdf