Histological study on the effect of histamine antagonists (ranitidine and promethazine) on the Distribution of collagen fibers in the uterus of the Pregnant rat on days 7 and 10 of pregnancy

Original article


Akram Y.Yasear 1 ; Abdel-Raoof A. Khatal 2; Ismeal A. Feitus 2

1-Department of oral Biology, Faculty of Dentistry, Garyounis University 2-Department of anatomy and Histology, Faculty of Medicine, Garyounis University, Benghazi, Libya.

Garyounis Medical Journal Vol. 21, No.1. 2004:55-63


Objective: To determine the effect of histamine antagonists promethazine (Hi blocker) and ranitidine (142 blocker) on distribution of collagen fibers in the pregnant rat uterus during the implantation period on days and 10 dpc (day post coitum). Material & methods: Sixty female rats of confirmed pregnancy have been divided in to two treated groups (48 rats) received intraperitoneally (i/p) promethazine and ranitidine respectively and one control group (12 rats) received normal saline via the same route. Histological technique was applied for sections taken from implantation sites at days 7 and 10 dpc.the sections were sustained in hematoxylin and eosin and Gomori one step trichrome stains. Results; Blastocysts were able to implant successfully and have initiated the implantation reaction and decidualization in the uteri of all rats used. As a normal reaction on day 7 dpc collagen fibers was found to be virtually absent in the decidual tissue of primary decidual zone (PDZ) and secondary decidual zone (SDZ )in contrast to undifferentiated zone(UDZ) in which the fibers was widely distributed. On day 10 dpc collagen fibers were abundant in the mesometrial decidual zone (MDZ). Collagen fibers were an important support to the blood vessels in this area which represents a prime route for establishment of nutrient supply through maternal blood vessels supplying the chorioallantoic placenta of rat. Conclusion: The results have revealed that i/p injection of histamine antagonists for Hi receptors (promethazine) and H2 receptor (ranitidine) did not interfere with the normal process of implantation of rat blastocyst.

Keywords: Histamine antagonists, implantation, rat, decidualization, collagen fibers