Intrinsic Innervation of Long Bones in Some Laboratory Animals

Original article


K. N Bhargava And C. C. Rao

Department of Anatomy, Faculty of Medicine, University of Garyounis, Benghazi, S.P.L.A.J.

Garyounis Medical Journal Vol. 3, No.2. July 1980:25-28


Innervation pattern in long bones in some laboratory animals such as rat, rabbit and guineapig was studied by a thiocholine technique apart from the usual methods such as silver impregnation and intra-vital/supravital methylene blue staining methods both in decalcified adult bones as well as in fresh frozen sections of growing bones. Using fresh decalcified frozen sections of young bones, the innervation could be more clearly visualised. By this method we could positively establish that the pattern of innervation follows closely the arterial pattern. The innervation of a growing bone is more profuse and suggest a direct control on the blood flow and influence on the growth and formation of the bone as suggested by Clavo (1968). Nerves were seen following the blood vessels to the epiphyseal centres of ossification which were also rich in enzyme activity. Nerves and nerve endings could be seen in profusion in the periosteum and in the synovial membrane as well. Nerves were studied in all the following three regions of a growing bone, namely, (1) Diaphysis (2) Epiphysis and (3) Epiphysio-metaphyseal junction.

Keywords: Intrinsic Innervation of Long Bones in Some Laboratory Animals