M. F. Abdel-Wahab 1, Y. M. Megahed 2, A. A. Ismail 2, A. A. Haftz 2, R. A. Khater 2
1-Department of Biochemistry, Faculty of Medicine, University of Garyounis, Benghazi S.P.L.A.J. 2-Department of Biochemistry, Faculty of Medicine, Cairo University, Egypt.
Garyounis Medical Journal Vol. 1, No.2. July 1978:27-30
L-β-phenylalanine was prepared as radioactive iodinated amino acid and purified on a Sephadex G-25 Gel-filtration column. A pH 9.3 buffer was used containing the amino acid glycine. The radioactive amino acid was tested for purity by Ekco scintillation counter and a Carl-Zeiss Spectrophotometer.
Twenty-two normal volunteers aged 20—35 years were given the test dose with a known weight of skimmed cheese and bread. Blood samples were taken at different time intervals and subjected to radioassay. The results were represented on graphs and interpreted in terms of radioactivity considering the balance between absorption and utilization.
Keywords: L-β-Phenylalanine-I125 as an Example of Amino Acids to Study the Absorption and Utilization of Proteins in Malnutrition