Long term formalin preserved stool specimens for detection of intestinal parasites from school aged children in Tripoli, Libya.

Original article

English

Ben Musa NA, Ibrahim R.

Department of Medical Parasitology, Faculty of Medicine, Al Fateh University, Tripoli, Libya. najla_bm@yahoo.com

J Egypt Soc Parasitol. 2007 Dec;37(3):1049-54.

Abstract

A total of 949 single stool samples were collected from school aged children (5-14 years old) in the city of Tripoli. The samples were preserved in 10% formalin and examined by routine microscopy using normal saline and Lugol’s iodine preparations as well as the formol ethyl concentration method after a storage period of twelve months at room temperature. Of 949 samples examined 4.5% were positive. Giardia lamblia and Entamoeba coli were the only protozoan parasites identified with an infection rate of 3.2% & 1.3% respecttively. No helminthes were detected in any of the samples. About 14% of the children had intestinal yeast infection Candida albicans in their stool of which 0.63% was infected with intestinal parasites. No distortion or alteration of morphology was observed particularly in G. lamblia. Preservation in 10% formalin is a very productive means for the accurate identification of protozoan parasites.

Keywords: Long term formalin preserved stool specimens for detection of intestinal parasites from school aged children in Tripoli, Libya.

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