Original article
English
Mohammed A. A. Khadre
Department of Microbiology & Parasitology Faculty of Medicine, University of Al Fateh Tripoli, The Socialist People’s Libyan Arab Jamahirija.
Al Fateh Med. J Vol .2, No. 1. July 1982:9-16
Abstract
The ability of pathogenic strain of Mycobacterium tuberculosis to grow in established cell lines of rabbit macrophages has been demonstrated both qualitatively and quantitatively. Photographs showing the intracellular growth of tubercle bacilli in rabbit macrophages are presented. The bacteria were able to grow in the cytoplasm and/or the nucleus. Growth started on day 3 post-inoculation. Cell destruction and degeneration started at day 7 post-inoculation and was complete at day 14. Quantitative evidence for growth of tubercle bacilli in rabbit macrophage cell lines is shown by the increase in numbers of bacteria to 185-fold in static cultures in 14 days and to 400-fold in agitated cultures in 23 days. The significance of these results is discussed.
Keywords: Propagation of Pathogenic Strains of Mycobacterium tuberculosis in Tissue Cultures of Rabbit Macrophages
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