Original article
English
Musa NB, Abdel Gawad MA.
Department of Medical Parasitology, Faculty of Medicine, Al Fatah University, Tripoli, Libya.
J Egypt Soc Parasitol. 2004 Apr;34(1):333-44.
Abstract
Three Babesia divergens isolates have been cultured continuously for 6 months in rat erythrocytes using the candle jar technique. One isolate was already rat-adapted, the other two became adapted to rats through continuous culturing in rat erythrocytes. Babesia was cultured in rat erythrocytes in RPMI medium supplemented with 20% foetal calf serum. The highest parasitaemia was 35% and multi-parasitization of red blood cells was often observed. Cultures of B. divergens remained infective to splenectomized rats. Cultures with high parasitaemias contained a large number of extra-cellular merozoites, when separated from the red blood cells, they retained their infectivity.
Keywords: Babesia divergens ,Babesiosis
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