Original article
English
A. A. Abdel-Gayoum
Department of Biochemistry Faculty of medicine Al-Arab Medical University Benghazi-Libya
Garyounis Medical Journal Vol.19. 1998-2002: 83-94
Abstract
The effect of gentamicin on the activity of Na, K, ATPase in crude preparation s of rat kidney cortex and liver was investigated in Vitro in the presence or absence of reduced glutatione (GSH) or ascorbate. The results revealed that gentamicin inhibited the cortical Na’ K ATPase by 10.6%, but failed to inhibit the liver enzyme. The sensitivity of the tissue-specific enzyme to gentamicin was in contrast to their reported affinities to the cardiac glycosides (Ouabain). The results also demonstrated failure of GSH to protect the Na, K ATPase against the antibiotic, whereas, ascorbate successfully protected the cortical enzyme against inhibition by gentamicin.
The findings suggest a possible role for the gentamicininduced inhibition of the kidney cortical Na, K, ATPase in the development of nephrotoxicity. The results also correlate between failure of GSH to protect the cortical enzyme from inhibition by gentamicin and its reported failure to protect against the gentamicin-induced nephrotoxicity. The protective effect of ascorbate to the cortical enzyme against the gentamicin challenge indicates a possible beneficial role for ascorbate in alleviation of the gentamicin-induced nephrotoxicity.
Keywords: Na, K , ATPase, Kidney, Cortex, Liver, Gentamicin, Rat
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