Original article
English
LM. Shweref 1, R.M. Sharrard 2 , J. Dame 3 P. Nathan, R. Ginsburg 3 , R. C. Rees 1,2 , Dr.C.W. Potter 1,2
1-Department of Experimental and Clinical Microbiology, University of Sheffield Medical School, Sheffield. 2-Oncogen Research Group, Institute for Cancer Studies, University of Sheffield Medical School, Sheffield. 3- Department of Obstetrics and Gynecology, Northern General Hospital, Sheffield.
LJMR Vol 1, No.1 2002: 53-63
Abstract
A total of 75 biopsy specimens from female genital tract, including wound tissue and tissues diagnosed histologically as condylomata acuminata, non-condylomatous virus infection, koilocytosis, CIN-I-III and squamous adenocarcinoma, were tested for human papillomaviruses (HPV) by in situ Hybridization. Selected blocks from this group were also tested for HPV 16 and 18 using the polymerase chain reaction (PCR). The results of in situ hybridization tests showed no detectable HPV sequence in normal tissue; HPV was detected in most cases of koilocytosis, non-condylomatous viral infection and condyloma acuminata; and HPV was detected in ten of the fourteen cases of the CIN and three of the eighteen cases of the adenocarcinoma. From these results nineteen specimens were tested by PCR: in no cases did PCR detect HPV sequences in tissue negative for these sequences by in situ hybridization; and blocks positive for type 6/11 or 31/33/35 were also negative by PCR. The results indicated good agreement between in situ hybridization and PCR, and suggest that the former test is adequate for routine testing of tissues.
Keywords: Detection Of Human Papilloma Virus In Cervical Lesions By In Situ Hybridization And Polymerase Chain Reaction
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